Difference between revisions of "Part:BBa K2835000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt and the AgeI site at 685 bp was changed to acgggt.
+
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
  
 
===Source===
 
===Source===

Revision as of 13:55, 29 September 2018


Enigneered mutant of mRFP1 + RBS (compatible with all iGEM RFC assembly standards)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.

Source

Discosoma striata (coral)

References