Difference between revisions of "Part:BBa K2762001"
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The rbcX part encode the the chaperon of rbcX RubisCO. The rbcX contribute to the correct folding of the whole RubisCO enzyme. | The rbcX part encode the the chaperon of rbcX RubisCO. The rbcX contribute to the correct folding of the whole RubisCO enzyme. | ||
===Biology=== | ===Biology=== | ||
| − | The rbcX genes are from cyanobacteria Synechococcus elongatus PCC 7002. We designed the T7 promoter (BBa_I719005)for the gene and cloned gene in BL21 to ensure the high expression rate. We also did the coden optimization for the gene to ensure successful expression. To get more information, see our composite part: | + | The rbcX genes are from cyanobacteria Synechococcus elongatus PCC 7002. We designed the T7 promoter (BBa_I719005) for the gene and cloned gene in BL21 to ensure the high expression rate. We also did the coden optimization for the gene to ensure successful expression. To get more information, see our composite part: BBa_K2762011. |
===Characterization=== | ===Characterization=== | ||
| − | We did two experiment to confirm the expression of this part. First we inserted the part on pSB1C3 and cloned the plasmid into DH5 alpha. We extracted the plasmid after the colony formed and did the enzyme digestion to confirm the insertion was successful. Second, we cloned the plasmid into BL21(DE3) and induced the promoter with IPTG. We than did the SDS PAGE to confirm the present of the RbcX and RbcS protein. | + | We did two experiment to confirm the expression of this part. First we inserted the part on pSB1C3 and cloned the plasmid into DH5 alpha. We extracted the plasmid after the colony formed and did the enzyme digestion to confirm the insertion was successful. Second, we cloned the plasmid into BL21 (DE3) and induced the promoter with IPTG. We than did the SDS-PAGE to confirm the present of the RbcX and RbcS protein. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:35, 29 September 2018
Assembly chaperone of ribulose-bisphosphate carboxylase/oxygenase rbcX
Usage
The rbcX part encode the the chaperon of rbcX RubisCO. The rbcX contribute to the correct folding of the whole RubisCO enzyme.
Biology
The rbcX genes are from cyanobacteria Synechococcus elongatus PCC 7002. We designed the T7 promoter (BBa_I719005) for the gene and cloned gene in BL21 to ensure the high expression rate. We also did the coden optimization for the gene to ensure successful expression. To get more information, see our composite part: BBa_K2762011.
Characterization
We did two experiment to confirm the expression of this part. First we inserted the part on pSB1C3 and cloned the plasmid into DH5 alpha. We extracted the plasmid after the colony formed and did the enzyme digestion to confirm the insertion was successful. Second, we cloned the plasmid into BL21 (DE3) and induced the promoter with IPTG. We than did the SDS-PAGE to confirm the present of the RbcX and RbcS protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]