Difference between revisions of "Part:BBa K2666000"
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The Montpellier iGEM team 2018 designed a construction that produce LL-37, an antimicrobial peptide from human macrophages and leukocytes in Lactobacillus jensenii (more information).  
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The Montpellier iGEM team 2018 designed a construction that produce and secrete LaM4, a nanobody against RFP and mcherry in Lactobacillus jensenii (more information). For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. The signal peptid is L. jensenii specific [2]. Figure 1 illustrates the detailed design.
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For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. This RpsU sequence also contains the putative sequence for the RBS. Figure 1 illustrates the detailed design.  
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Figure 1 : Construct design. The sequence that encode LL-37 with a strong promoter RpsU.
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Figure 1 : Construct design. The sequence that encode scFv LaM-4 with a SP CsbA and produce by a strong promoter RpsU.
  
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LL-37 has shown effect in vivo in mice. Females naturally cycling to estrous phase failed to become pregnant when in injected with LL-37 and sperm. LL-37 also showed in vitro effect on human sperm which makes it a very good potential peptide for contraception. [2]
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LaM4 is used as a control [3]. It is an antibody against RFP & mcherry. This control allow us to check the efficiency of the promoter and the signal peptid.

Revision as of 08:10, 29 September 2018

The Montpellier iGEM team 2018 designed a construction that produce and secrete LaM4, a nanobody against RFP and mcherry in Lactobacillus jensenii (more information). For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. The signal peptid is L. jensenii specific [2]. Figure 1 illustrates the detailed design.


Figure 1 : Construct design. The sequence that encode scFv LaM-4 with a SP CsbA and produce by a strong promoter RpsU.

LaM4 is used as a control [3]. It is an antibody against RFP & mcherry. This control allow us to check the efficiency of the promoter and the signal peptid.