Difference between revisions of "Part:BBa K2555003"

 
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<partinfo>BBa_K2555003 short</partinfo>
 
<partinfo>BBa_K2555003 short</partinfo>
  
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This is BBa_K2555003 which used to test value of CueR used in BBa_K2555003.
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BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP and Terminator. This part is used to indirectly the amount of CueR protein in Part BBa_K2555004 expression in our project.
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The plasmid including araC, araBAD promoter, RBS, and superfolder GFP.
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When the Ara enter the part, it will combined with araC, which active araBAD combining with RBS to produce CueR. Then the super folder GFP will generate a certain amount of fluorescence, which can be easily observed. Through this way, we can know the amount of CueR generated.
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Revision as of 06:56, 29 September 2018


pBAD-RBS-sfGFP

BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP and Terminator. This part is used to indirectly the amount of CueR protein in Part BBa_K2555004 expression in our project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067
    Illegal SapI.rc site found at 1355