Difference between revisions of "Part:BBa K2560256:Design"

 
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===Design Notes===
 
===Design Notes===
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The part contains the whole coding region from the <i>birA</i> gene of <i>C. glutamicum</i> (NCgl0679) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).
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If <i>birA</i> shall be used with a 5' tag use this part, if there is no need for tagging, use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560253 BBa_K2560253]. <br>
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GGTCTCG<b>GATG</b>-coding_region-<b>GCTT</b>TGAGACC
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The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048.
  
  
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Accession number of encoded protein: NP_599941.1
 
Accession number of encoded protein: NP_599941.1
  
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<i>birA</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI  
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<i>birA</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560002 BBa_K2560002]via BsmBI  
  
  
 
===References===
 
===References===
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Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765
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Samols D., Thornton CH., Murtif VL., Kumar GK., Haase FC., Wood HG. (1988). Evolutionary conservation among biotin enzymes. J. Biol. Chem. 263. 6461-6464.

Revision as of 19:23, 28 September 2018


birA from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the birA gene of C. glutamicum (NCgl0679) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

If birA shall be used with a 5' tag use this part, if there is no need for tagging, use BBa_K2560253.

GGTCTCGGATG-coding_region-GCTTTGAGACC


The sequence was codonoptimized for V. natriegens ATCC 14048.


Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0679

Accession number of encoded protein: NP_599941.1

birA was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002via BsmBI


References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765

Samols D., Thornton CH., Murtif VL., Kumar GK., Haase FC., Wood HG. (1988). Evolutionary conservation among biotin enzymes. J. Biol. Chem. 263. 6461-6464.