Difference between revisions of "Part:BBa K2560252"

Revision as of 18:00, 28 September 2018

accD subunit from C. glutamicum, codonoptimized for V. natriegens

accD encodes the carboxytransferase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).

For this part, accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

If accD shall be used with an 5' tag use BBa_K2560255, if there is no need for tagging, use this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 768
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 277
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2560252 short</partinfo>
-accD encodes the carboxytransferase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA). For this part, accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI. If accD shall be used with an 5' tag use BBa_K2560255, if there is no need for tagging, use this part.
+[[Image:BBa K2560251 Acc subunits Cheng et al 2016.png|400px|right|Acetyl-CoA Carboxylase subunits]]
+<i>accD</i> encodes the carboxytransferase domain of the acetyl-CoA-carboxylase (Acc) in <i>C. glutamicum</i>. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In <i>C. glutamicum</i> the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).
+For this part, <i>accD</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002] via BsmBI.
+If <i>accD</i> shall be used with an 5' tag use [https://parts.igem.org/Part:BBa_K2560255 BBa_K2560255], if there is no need for tagging, use this part.
 <!-- Add more about the biology of this part here