Difference between revisions of "Part:BBa K2688026:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This plasmid was obtained following PCR mutagenesis of pSB1C3-LEE5_gfp_variant-1_inframe (BBa_K2688016) in order to delete the open reading frame corresponding to a fragment of tir gene.
+
This plasmid was obtained following PCR mutagenesis of pSB1C3-LEE5_gfp_variant-1_inframe (BBa_K2688016) in order to delete the open reading frame corresponding to a fragment of tir gene, which yielded BBa_K2688017. The introduced point mutation was then PCR corrected, yielding this biobrick.
  
  

Latest revision as of 16:11, 28 September 2018


LEE5_native_ΔTir


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 50


Design Notes

This plasmid was obtained following PCR mutagenesis of pSB1C3-LEE5_gfp_variant-1_inframe (BBa_K2688016) in order to delete the open reading frame corresponding to a fragment of tir gene, which yielded BBa_K2688017. The introduced point mutation was then PCR corrected, yielding this biobrick.



Source

LEE5 is a topic of research at the host lab, which provided us with the source plasmid pKK-LEE5-gfp.

References