Difference between revisions of "Part:BBa K2688026"

 
 
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<partinfo>BBa_K2688026 short</partinfo>
  
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LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon.
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Compared to LEE5_native (BBa_K2688010), this variant has has a deletion that removes the tir fragment. (The beginning of an ORF is present in the native sequence : it is removed for improved expression and to avoid clash of reading frame)
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This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017). 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2688026 SequenceAndFeatures</partinfo>

Latest revision as of 16:10, 28 September 2018

LEE5_native_ΔTir

LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon.


Compared to LEE5_native (BBa_K2688010), this variant has has a deletion that removes the tir fragment. (The beginning of an ORF is present in the native sequence : it is removed for improved expression and to avoid clash of reading frame)


This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 50