Difference between revisions of "Part:BBa K2560251:Design"

(→‎Design Notes)
(→‎Source)
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Source of the part:
 
Source of the part:
 +
 
Genome: <i>Corynebacterium glutamicum</i> ATCC 13032
 
Genome: <i>Corynebacterium glutamicum</i> ATCC 13032
 +
 
Accession number of gene: NCgl0670
 
Accession number of gene: NCgl0670
 +
 
Accession number of encoded protein: NP_599932.1
 
Accession number of encoded protein: NP_599932.1
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<i>accBC</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560002 BBa_K2560002] via BsaBI.
+
 
 +
<i>accBC</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560002 BBa_K2560002] via BsmBI
  
 
===References===
 
===References===
 
Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765
 
Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765

Revision as of 15:40, 28 September 2018


accBC subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). If accBC shall be used with an 5' tag use BBa_K2560254, if there is no need for tagging, use this part.
AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA

The sequence was codonoptimized for V. natriegens ATCC 14048.

Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0670

Accession number of encoded protein: NP_599932.1

accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI

References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765