Difference between revisions of "Part:BBa K2560254"

Revision as of 15:36, 28 September 2018

accBC subunit from C. glutamicum, codonoptimized for V. natriegens

accBC encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).

For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

If accBC shall be used with an 5' tag use this part, if there is no need for tagging, use BBa_K2560251.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1142
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 423
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <i>accBC</i> encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in <i>C. glutamicum</i>. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In <i>C. glutamicum</i> the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).
-For this part, accBC was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002] via BsaBI.
+For this part, accBC was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002] via BsmBI.
 If <i>accBC</i> shall be used with an 5' tag use this part, if there is no need for tagging, use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560251 BBa_K2560251].