Difference between revisions of "Part:BBa K2560254"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | If <i>accBC</i> shall be used with an 5' tag use this part, if there is no need for tagging, use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560251 BBa_K2560251]. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:54, 28 September 2018
accBC subunit from C. glutamicum, codonoptimized for V. natriegens
accBC encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).
For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsaBI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1142
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 423
- 1000COMPATIBLE WITH RFC[1000]