Difference between revisions of "Part:BBa K2560254"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2560254 short</partinfo>
 
<partinfo>BBa_K2560254 short</partinfo>
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[[Image:BBa K2560251 Acc subunits Cheng et al 2016.png|400px|right|Acetyl-CoA Carboxylase subunits]]
 
[[Image:BBa K2560251 Acc subunits Cheng et al 2016.png|400px|right|Acetyl-CoA Carboxylase subunits]]

Revision as of 14:52, 28 September 2018


accBC subunit from C. glutamicum, codonoptimized for V. natriegens

Acetyl-CoA Carboxylase subunits

accBC encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).

For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsaBI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]