Difference between revisions of "Part:BBa K2560251:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site.
+
The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites.
 
AACGTCTCGCTCG<b>AATG</b>-coding_region-<b>GCTT</b>TGAGGGAGACGAA
 
AACGTCTCGCTCG<b>AATG</b>-coding_region-<b>GCTT</b>TGAGGGAGACGAA
  

Revision as of 14:22, 28 September 2018


accBC subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites. AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA

The sequence was codonoptimized for V. natriegens ATCC 14048.

Source

Source of the part: Genome: Corynebacterium glutamicum ATCC 13032 Accession number of gene: NCgl0670 Accession number of encoded protein: NP_599932.1 accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsaBI.

References