Difference between revisions of "Part:BBa K2835001"
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<partinfo>BBa_K2835001 short</partinfo> | <partinfo>BBa_K2835001 short</partinfo> | ||
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| + | The protein coding sequence for iGEM11_Uppsala's highly engineered mutant RFP with strong RBS made compatible with the iGEM RFC 25 assembly standard. and thereby all iGEM assembly standards. This improvement was made by iGEM18_Stockholm . | ||
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| + | [[Image:Rfpminiprep.jpeg|300px]] | ||
| + | [[Image:BothRFP.jpeg|300px]] | ||
The colonies will turn red after 18 hours. | The colonies will turn red after 18 hours. | ||
Revision as of 07:29, 28 September 2018
Constitutive reporter: strong promoter + RBS + mutant of mRFP1 (RFC25 compatible)
The protein coding sequence for iGEM11_Uppsala's highly engineered mutant RFP with strong RBS made compatible with the iGEM RFC 25 assembly standard. and thereby all iGEM assembly standards. This improvement was made by iGEM18_Stockholm .
The colonies will turn red after 18 hours.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]