Difference between revisions of "Part:BBa K2886008:Design"
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
This part is used as a positive control of our experiment because it could emit fluorescence. even without the ligand VEGF. And it should used with BBa_K2886009 | This part is used as a positive control of our experiment because it could emit fluorescence. even without the ligand VEGF. And it should used with BBa_K2886009 | ||
| + | The actual sequence of this part is different from the sequence page.Delete the scar and inset the sequence ggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggctctttgtgttggaggcggaggctcc and ggaggcggaggctcc between BBa_K2886002 & BBa_K2886006 and BBa_K2886006 & BBa_K2886004 respectively. | ||
Latest revision as of 04:29, 28 September 2018
VEGF-scFv-LEUCINE_ZIPPER-cECFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 524
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is used as a positive control of our experiment because it could emit fluorescence. even without the ligand VEGF. And it should used with BBa_K2886009 The actual sequence of this part is different from the sequence page.Delete the scar and inset the sequence ggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacgacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggctctttgtgttggaggcggaggctcc and ggaggcggaggctcc between BBa_K2886002 & BBa_K2886006 and BBa_K2886006 & BBa_K2886004 respectively.
Source
It is a composite part made from BBa_K2886002, BBa_K2886006 and BBa_K2886004.