Difference between revisions of "Part:BBa K2669001"

Revision as of 10:14, 27 September 2018

Bilirubin-inducible UnaG+6xHis-tag+Flexible linker

WORK IN PROGRESS

This part is a correction of the Uppsala 2016’s Part:BBa_K2003011, which we noticed had a misplaced start codon. This misplaced start codon caused two major problems:

1) This would lead to a reduction in UnaG expression due to a large space being created between the RBS and the start codon

2) This would lead to no histidine tag being expressed

We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part Part:BBa_K2003011.

UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments, or potentially in environments where bilirubin is naturally present, such as the intestines.

Sequence and Features