Difference between revisions of "Part:BBa K2568006:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We added kozak sequence to enhance translation efficiency and protein expression,and added FLAG-tag to help us conduct subsequent experimental testing.
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We added kozak sequence to make proteins fold correctly ,and FLAG-tag to help us conduct subsequent experimental testing,and signal peptide sequende to enhance the transfection efficiency and protein expression.
  
  

Latest revision as of 04:04, 27 September 2018


Virus receptor Nectin 4 with Kozak sequence and FLAG-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 220
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1041
    Illegal BsaI.rc site found at 1369
    Illegal SapI.rc site found at 1168


Design Notes

We added kozak sequence to make proteins fold correctly ,and FLAG-tag to help us conduct subsequent experimental testing,and signal peptide sequende to enhance the transfection efficiency and protein expression.


Source

Referring to the canine-derived NECTIN4 mRNA sequence (GenBank accession number: NM_001313853.1) registered in GenBank, we optimized the coding sequence and added the necessary sequences.

References