Difference between revisions of "Part:BBa K2560251"
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<partinfo>BBa_K2560251 short</partinfo> | <partinfo>BBa_K2560251 short</partinfo> | ||
| + | [[Image:BBa K2560251 Acc subunits Cheng et al 2016.png|400px|right|Acetyl-CoA Carboxylase subunits]] | ||
<i>accBC</i> encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in <i>C. glutamicum</i>. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In <i>C. glutamicum</i> the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA). | <i>accBC</i> encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in <i>C. glutamicum</i>. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In <i>C. glutamicum</i> the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA). | ||
Revision as of 20:27, 26 September 2018
accBC subunit from C. glutamicum, codonoptimized for V. natriegens
accBC encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).
For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsaBI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1142
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 423
- 1000COMPATIBLE WITH RFC[1000]