Difference between revisions of "Part:BBa K2560251:Design"

 
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===Design Notes===
 
===Design Notes===
−
The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site.
+
The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site.
 
AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA
 
AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA
  
−
The sequence was codonoptimized for V. natriegens ATCC 14048.
+
The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048.
  
  
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Source of the part:
 
Source of the part:
−
Genome: Corynebacterium glutamicum ATCC 13032
+
Genome: <i>Corynebacterium glutamicum</i> ATCC 13032
 
Accession number of gene: NCgl0670
 
Accession number of gene: NCgl0670
 
Accession number of encoded protein: NP_599932.1
 
Accession number of encoded protein: NP_599932.1
−
accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector ****** via BsaBI.
+
<i>accBC</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560002 via BsaBI.
  
 
===References===
 
===References===

Revision as of 19:14, 26 September 2018


accBC subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site. AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA

The sequence was codonoptimized for V. natriegens ATCC 14048.


Source

Source of the part: Genome: Corynebacterium glutamicum ATCC 13032 Accession number of gene: NCgl0670 Accession number of encoded protein: NP_599932.1 accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560002 via BsaBI.

References