Difference between revisions of "Part:BBa K2656200"
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− | Backbone pSB1C3 modified to | + | Backbone pSB1C3 modified to receive transcriptional units created in alpha 1 plasmids of Golden Braid 3.0 in a Golden Gate reaction using BsmbI endonuclease. It contains a red transcriptional unit in the cloning site for the screening. |
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Revision as of 15:27, 26 September 2018
pBIOBRICKATOR
Backbone pSB1C3 modified to receive transcriptional units created in alpha 1 plasmids of Golden Braid 3.0 in a Golden Gate reaction using BsmbI endonuclease. It contains a red transcriptional unit in the cloning site for the screening.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 5
Plasmid lacks a suffix.
Illegal SpeI site found at 921
Illegal PstI site found at 935 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5
Illegal NheI site found at 44
Illegal NheI site found at 67
Illegal SpeI site found at 921
Illegal PstI site found at 935
Illegal NotI site found at 11
Illegal NotI site found at 928 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5
Illegal XhoI site found at 1952
Illegal XhoI site found at 2844 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5
Illegal suffix found at 921 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5
Plasmid lacks a suffix.
Illegal XbaI site found at 20
Illegal SpeI site found at 921
Illegal PstI site found at 935
Illegal AgeI site found at 653
Illegal AgeI site found at 765 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.