Difference between revisions of "Part:BBa K2810011:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
This part was synthesised as a gBlock and was not put together from separate level 0 parts. This means unlike our other parts in the collection, it does NOT have the Type IIS restriction enzyme sites and sticky ends that make it GoldenGate compatible.
+
This part was synthesised as a gBlock and was not put together from separate level 0 parts. This means unlike our other parts in the collection, it does have the Type IIS restriction enzyme sites and sticky ends making it GoldenGate compatible, but it was not made using GoldenGate cloning.
  
  
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===Source===
 
===Source===
  
−
Sequenced as a gBlock. The promoter is from X, the coding sequence originally RFP from <i>Discosoma</i>, and the promoter from X.
+
Sequenced as a gBlock. The promoter is from BBa_J23100, RBS from BBa_B0030, and the coding sequence originally RFP from <i>Discosoma</i>, and the promoter from BBa_B0015.
  
 
===References===
 
===References===

Latest revision as of 10:24, 26 September 2018


mCherry under control of the bacterial promoter and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was synthesised as a gBlock and was not put together from separate level 0 parts. This means unlike our other parts in the collection, it does have the Type IIS restriction enzyme sites and sticky ends making it GoldenGate compatible, but it was not made using GoldenGate cloning.


Source

Sequenced as a gBlock. The promoter is from BBa_J23100, RBS from BBa_B0030, and the coding sequence originally RFP from Discosoma, and the promoter from BBa_B0015.

References