Difference between revisions of "Part:BBa K2669000"

Revision as of 14:39, 25 September 2018

Strongly constitutive His-tagged+flexible linker UnaG

UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments or even potentially in environments where bilirubin is naturally present, such as the intestines. While researching the previous work from the iGEM Uppsala 2016 team, we noticed that their part (BBa_K2003011) had an error in it (a misplaced start codon) which would lead to not only reduced UnaG expression but also not include the Histidine tag their part should be expressing according to the igem registry!

We decided that we would rectify this issue by engineering a composite part with the start codon in the correct position and compare it to the previous part (BBa_K2003011). We designed this composite part by combining a strong promoter (BBa_J23119), a strong RBS (BBa_J34801), and a double terminator (BBa_B0014) around the modified UnaG part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2669000 short</partinfo>
-UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments, or potentially in environments where bilirubin is naturally present, such as the intestines. While researching the previous work from the iGEM Uppsala 2016 team, we noticed that their part (BBa_K2003011) had an error in it (a misplaced start codon) which would lead to not only reduced UnaG expression but also not include the Histidine tag their part should be expressing according to the igem registry!
+UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments or even potentially in environments where bilirubin is naturally present, such as the intestines. While researching the previous work from the iGEM Uppsala 2016 team, we noticed that their part (BBa_K2003011) had an error in it (a misplaced start codon) which would lead to not only reduced UnaG expression but also not include the Histidine tag their part should be expressing according to the igem registry!
 We decided that we would rectify this issue by engineering a composite part with the start codon in the correct position and compare it to the previous part (BBa_K2003011).  We designed this composite part by combining a strong promoter (BBa_J23119), a strong RBS (BBa_J34801), and a double terminator (BBa_B0014) around the modified UnaG part.