Difference between revisions of "Part:BBa K2560007:Design"
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The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning. | The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning. | ||
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===Source=== | ===Source=== |
Revision as of 11:17, 25 September 2018
Phytobrick version of BBa_J23100
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning.
"
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector (BBa_K2560002) using Golden Gate assembly.
Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT
Reverse Oligo: CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC