Difference between revisions of "Part:BBa K2560007:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
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More information coming soon!
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Forward Oligo:
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CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT
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Reverse Oligo:
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CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC
  
 
===References===
 
===References===

Revision as of 08:26, 25 September 2018


Phytobrick version of BBa_J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was obtained from part BBa_J23100. The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector (BBa_K2560002) using Golden Gate assembly.

Source

Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT

Reverse Oligo: CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC

References