Difference between revisions of "Part:BBa K2598011"
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<partinfo>BBa_K2598011 short</partinfo> | <partinfo>BBa_K2598011 short</partinfo> | ||
− | This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It | + | This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It contains a DNA-binding loop and is able to combine to non-active T7 RNAP core fragment (BBa_K2598001) and direct it to specific promoter pCGG(BBa_K2598023) |
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Revision as of 07:59, 25 September 2018
T7 RNAP sigma fragment CGG
This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It contains a DNA-binding loop and is able to combine to non-active T7 RNAP core fragment (BBa_K2598001) and direct it to specific promoter pCGG(BBa_K2598023)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 578
Illegal NgoMIV site found at 1193
Illegal NgoMIV site found at 1202 - 1000COMPATIBLE WITH RFC[1000]