Difference between revisions of "Part:BBa K2598011"

 
Line 3: Line 3:
 
<partinfo>BBa_K2598011 short</partinfo>
 
<partinfo>BBa_K2598011 short</partinfo>
  
This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It can combine to non-active T7 RNAP core fragment (BBa_K2598001) and direct it to specific promoter pCGG(BBa_K2598023)
+
This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It contains a DNA-binding loop and is able to combine to non-active T7 RNAP core fragment (BBa_K2598001) and direct it to specific promoter pCGG(BBa_K2598023)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:59, 25 September 2018


T7 RNAP sigma fragment CGG

This is a T7 RNAP sigma pragment obtained by Segallshapiro T H et al. It contains a DNA-binding loop and is able to combine to non-active T7 RNAP core fragment (BBa_K2598001) and direct it to specific promoter pCGG(BBa_K2598023)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 578
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1202
  • 1000
    COMPATIBLE WITH RFC[1000]