Difference between revisions of "Part:BBa K2599000"
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'''''Figure 1:SDS-PAGE gels of TDPs after protein purification.'''
 
'''''Figure 1:SDS-PAGE gels of TDPs after protein purification.'''

Revision as of 15:47, 24 September 2018


Antimicrobial peptide - Subtilosin

This biobrick is the basic part of the Subtilosin, more information please look for the composite part (BBa_K2599008).



Figure 1:SDS-PAGE gels of TDPs after protein purification.


Introduction

Bacillus subtilis produces a bacteriocin called Subtilosin that possesses antibacterial activity against certain gram-positive bacteria. The bacteriocins are a group of anitmicrobial peptides that are often distinguished from traditioinal antibiotics by their narrow range of avtivity against closely related bacteria.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Subtilosin sequence length is around 147 b.p.


Figure 2 PCR




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference