Difference between revisions of "Part:BBa K2688020"
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Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island. | Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island. | ||
Our results show that Ler1 can be used to generate heterogeneity of LEE5 promoter (see BBa_K2688016 and BBa_K2688018) activity within E. coli population. Under some conditions (e.g. 50 ng/ml anhydrotetracyclin), a bimodal expression of LEE5 can be observed in E. coli harbouring pSB1C3-tetO-RBS-ler1 and an integrated copy of LEE5-gfp. | Our results show that Ler1 can be used to generate heterogeneity of LEE5 promoter (see BBa_K2688016 and BBa_K2688018) activity within E. coli population. Under some conditions (e.g. 50 ng/ml anhydrotetracyclin), a bimodal expression of LEE5 can be observed in E. coli harbouring pSB1C3-tetO-RBS-ler1 and an integrated copy of LEE5-gfp. | ||
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| + | <partinfo>BBa_K2688020 SequenceAndFeatures</partinfo> | ||
Latest revision as of 16:06, 23 September 2018
ler_1
Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island.
Our results show that Ler1 can be used to generate heterogeneity of LEE5 promoter (see BBa_K2688016 and BBa_K2688018) activity within E. coli population. Under some conditions (e.g. 50 ng/ml anhydrotetracyclin), a bimodal expression of LEE5 can be observed in E. coli harbouring pSB1C3-tetO-RBS-ler1 and an integrated copy of LEE5-gfp.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]