Difference between revisions of "Part:BBa K2688010"

Latest revision as of 14:20, 23 September 2018

LEE5_native

LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5.

This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 52

Revision as of 14:20, 23 September 2018 (view source) Briand (Talk \| contribs) ← Older edit		Latest revision as of 14:20, 23 September 2018 (view source) Briand (Talk \| contribs) m
Line 1:		Line 1:
	<partinfo>BBa_K2688010 short</partinfo>		<partinfo>BBa_K2688010 short</partinfo>
		+
		+
	LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5.		LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5.