Difference between revisions of "Part:BBa K2688012"
 
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LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5, with a generic GFP.
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When the protein Ler is present, this displays heterogeneneity of expression level, thus varying fluorescence level in each cell. (characterized by flow cytometry)
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Latest revision as of 14:13, 23 September 2018

LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5, with a generic GFP.

When the protein Ler is present, this displays heterogeneneity of expression level, thus varying fluorescence level in each cell. (characterized by flow cytometry)


LEE5_GFP_native


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 566
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 52
    Illegal BsaI.rc site found at 1209