Difference between revisions of "Part:BBa K2688010"
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This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).
 
This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2688000 SequenceAndFeatures</partinfo>

Revision as of 09:47, 23 September 2018

LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, named LEE5.

This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
    Illegal NgoMIV site found at 745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 844