Difference between revisions of "Part:BBa K2638101:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
removal of restriction sites
+
Removal of the restriction sites EcoRI and PstI by substitution of cytosine at position 363 with thymine and by substitution of the codon CTG at positions 1186 to 1188 with TTA
  
  
Line 16: Line 16:
  
 
===References===
 
===References===
 +
 +
Bieger, B., & Essen, L. O. (2001). Crystal structure of the catalytic core component of the alkylhydroperoxide reductase AhpF from Escherichia coli1. Journal of molecular biology, 307(1), 1-8.

Revision as of 21:00, 22 September 2018


alkyl hydroperoxide reductase, F52a subunit (ahpF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
    Illegal AgeI site found at 712
    Illegal AgeI site found at 964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 613


Design Notes

Removal of the restriction sites EcoRI and PstI by substitution of cytosine at position 363 with thymine and by substitution of the codon CTG at positions 1186 to 1188 with TTA


Source

Genome of Escherichia coli K-12 DH5alpha

References

Bieger, B., & Essen, L. O. (2001). Crystal structure of the catalytic core component of the alkylhydroperoxide reductase AhpF from Escherichia coli1. Journal of molecular biology, 307(1), 1-8.