Difference between revisions of "Part:BBa K2638100:Design"
 
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===Design Notes===
 
===Design Notes===
−
removal of restriction sites PstI and EcoRI
+
Removal of the restriction sites EcoRI and PstI by substitution of cytosine at position 63 with thymine and substitution of guanosine at position 186 with adenosine
  
  

Latest revision as of 20:39, 22 September 2018


alkyl hydroperoxide reductase, C22 subunit (ahpC)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of the restriction sites EcoRI and PstI by substitution of cytosine at position 63 with thymine and substitution of guanosine at position 186 with adenosine


Source

genome of Escherichia coli K-12 DH5alpha

References

Seaver, L. C., & Imlay, J. A. (2001). Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. Journal of bacteriology, 183(24), 7173-7181.