Difference between revisions of "Part:BBa K2656013"
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===Usage and Biology===
 
===Usage and Biology===
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We have used this CDS to create 5 different transcriptional units with the same promoter (<a id=https://parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a> and terminator (<a id=https://parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a>) to test the relative strength of our 5 different RBS.
 
  
 
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Revision as of 12:51, 22 September 2018


sfGFP Coding Sequence

Part BBa_I746916 domesticated to the Golden Braid 3.0 grammar. It can be used to assembly transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14