Difference between revisions of "Part:BBa M0050"

Revision as of 17:42, 5 December 2007

AANDENYALAA. (Very fast) SsrA degradation tag.

This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~5 Vmax/ [Clpx6] min-1 from (1), ~0.5%/min on log scale from (2), ~1 min half life from (3).

See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_M0050 short</partinfo>
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 This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease.
 The following rates of degradation of this tag are pulled from the corresponding references below:
-~5 Vmax/ [Clpx6] min-1    from (1)
+~5 Vmax/ [Clpx6] min-1    from (1),
-~0.5%/min  on log scale   from (2)
+~0.5%/min  on log scale   from (2),
-~1 min half life          from (3)
+~1 min half life          from (3).
 See the following references for further information on degradation rates and mechanisms of this tag: