Difference between revisions of "Part:BBa K2599000"

Revision as of 09:06, 18 September 2018

Antimicrobial peptide - Subtilosin

This biobrick is the basic part of the Subtilosin, more information please look for the composite part (BBa_K2599008).

Figure 1 biobrick picture

Introduction

Bacillus subtilis produces a bacteriocin called Subtilosin that possesses antibacterial activity against certain gram-positive bacteria. The bacteriocins are a group of anitmicrobial peptides that are often distinguished from traditioinal antibiotics by their narrow range of avtivity against closely related bacteria.

Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Subtilosin sequence length is around 147 b.p.

Figure 2 PCR

Reference

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 17: / Line 17: @@
 ===Cloning===
-We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Subtilosin sequence length is around 147 b.p. For the composite part, the sequence length should be near at 1191 b.p.
+We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Subtilosin sequence length is around 147 b.p.
@@ Line 24: / Line 24: @@
-===Expressing===
-We chose <i> E. coli </i> 2566 strain to express our antibacterial peptides. The expression of Subtilosin fused with intein was induced by IPTG in <i> E. coli </i>, and intein-subtilosin specifically bound to the column through chitin binding domain would be purified.
-Figure 3 SDS