Difference between revisions of "Part:BBa K2632009"
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We use BBa_J36850( Lpp-OmpA(46-159)-Streptavidin wild-type) to do the improvement. We add the RGD sequence at the same position of that in Salmonella, which can display the RGD on the outside of OmpA. | We use BBa_J36850( Lpp-OmpA(46-159)-Streptavidin wild-type) to do the improvement. We add the RGD sequence at the same position of that in Salmonella, which can display the RGD on the outside of OmpA. | ||
+ | <h3>Reference</h3> | ||
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+ | Park, S. H. et al. RGD peptide cell-surface display enhances the targeting and therapeutic efficacy of attenuated salmonella-mediated cancer therapy. Theranostics 6, 1672–1682 (2016).</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 12:07, 16 September 2018
OmpA coding sequence in Salmonella(Para-OmpA-RGD).
We use BBa_J36850( Lpp-OmpA(46-159)-Streptavidin wild-type) to do the improvement. We add the RGD sequence at the same position of that in Salmonella, which can display the RGD on the outside of OmpA.
Reference
Park, S. H. et al. RGD peptide cell-surface display enhances the targeting and therapeutic efficacy of attenuated salmonella-mediated cancer therapy. Theranostics 6, 1672–1682 (2016).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 293
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]