Difference between revisions of "Part:BBa K2632007"

 
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<partinfo>BBa_K2632007 short</partinfo>
 
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Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of Gasdermin D(&#21152;&#19968;&#20010;&#36229;&#38142;&#25509;&#21040;&#30456;&#20851;part &#30340;&#25551;&#36848;) acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD(pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. A377D mutation of full length Gasdermin D reduces the cell toxicity.
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Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of  <a href="https://parts.igem.org/Part:BBa_K2632003">Gasdermin D</a>  acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD(pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. A377D mutation of full length Gasdermin D reduces the cell toxicity.
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<h3>Reference</h3>
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<p>
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Ding J, Wang K, Liu W, et al. Pore-forming activity and structural autoinhibition of the gasdermin family[J]. Nature, 2016, 535(7610):111.
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===Usage and Biology===
 
===Usage and Biology===
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Revision as of 11:59, 16 September 2018

A377D mutant of full length Gasdermin D

Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of Gasdermin D acts as a effector of pyroptosis. Full length Gasdermin D is cleaved by Caspase 1 then release the PFD(pore-forming domain) which can oligomerize on the cell membrane. Formation of pore causes cell swelling, rupture of the membrane and massive leakage of cytosolic contents. A377D mutation of full length Gasdermin D reduces the cell toxicity.

Reference

Ding J, Wang K, Liu W, et al. Pore-forming activity and structural autoinhibition of the gasdermin family[J]. Nature, 2016, 535(7610):111.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 883