Difference between revisions of "Part:BBa K2599008"

Revision as of 10:42, 16 September 2018

T7 Promoter+RBS+Subtilosin+intein+CBD

NCTU_Formosa 2018 designed a composite part encoding the Subtilosin A sequence (BBa_K2599000), and then combined with a T7 promoter (BBa_I712074), a ribosome binding site (BBa_B0034), intein and chintin binding domain (CBD).

Figure 1 biobrick picture

Introduction

Subtilosin A is produced by Bacillus subtilis and belongs to the bacteriocin class V family. Bacteriocins are proteinaceous antimicrobial substances that are often distinguished from traditional antibiotics by their narrow range of avtivity against closely related bacteria. It had bacteriocidal activity against some gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium.

Structure Analysis

The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure.

Figure 2 structure picture

Sequence and Features