Difference between revisions of "Part:BBa K2599008"

Revision as of 10:38, 16 September 2018

T7 Promoter+RBS+Subtilosin+intein+CBD

NCTU_Formosa 2018 designed a composite part encoding the Subtilosin A sequence (BBa_K2599000), and then combined with a T7 promoter (BBa_I712074), a ribosome binding site (BBa_B0034), intein and chintin binding domain (CBD).

Figure 1 biobrick picture

Introduction

Subtilosin A is an antimicrobial peptide produced by Bacillus subtilis and belongs to the bacteriocin class V family. It had bacteriocidal activity against some gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium.

Structure Analysis

The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure.

Figure 2 structure picture

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1055
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 778
Illegal AgeI site found at 868
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 698

@@ Line 12: / Line 12: @@
 ===Introduction===
-Subtilosin A is an antimicrobial peptide produced by <i>Bacillus subtilis</i>. It had bacteriocidal activity against some gram-positive bacteria.
+Subtilosin A is an antimicrobial peptide produced by <i>Bacillus subtilis</i> and belongs to the bacteriocin class V family. It had bacteriocidal activity against some gram-positive bacteria such as <i>Listeria</i>, some species of <i>Bacillus</i> and <i>E.faecium</i>.
-===Structure analysis===
-The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure. The production of subtilosin A begins at the end of vegetative growth and finishes before spore formation.
+===Structure Analysis===
+The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure.
 Figure 2 structure picture