Difference between revisions of "Part:BBa K2632001"
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pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is FAD which is normal in nearly all organisms.
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Gene ent located in the cluster of enterobactin (ent) genes. In respond to iron deprivation, Salmonella enterica serovar Typhimurim secretes siderophores and enterobactin in SCV condictiont to sense and adapt to the intracellular environment of different types of host cells
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This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag
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<a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381001</a>.It's function is proved by parts                   
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<a href="https://parts.igem.org/Part:BBa_K2381017">BBa_K2381017</a>
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<br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2381000 parameters</partinfo>
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<partinfo>BBa_K2632001 parameters</partinfo>
 
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Revision as of 14:01, 15 September 2018

pMagFast

Gene ent located in the cluster of enterobactin (ent) genes. In respond to iron deprivation, Salmonella enterica serovar Typhimurim secretes siderophores and enterobactin in SCV condictiont to sense and adapt to the intracellular environment of different types of host cells

Reference

Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]