Difference between revisions of "Part:BBa K2555000"

Revision as of 13:52, 14 September 2018

PcopA-RBS-RiboJ-sfGFP

BBa_K2555000 including the promoter of cop A, ribosome binding site, self-cleaving RNA ribozyme RiboJ, and protein coding region-sfGFP. In E.coli. As a ATPase, cop A is regulated by a copper-responsive protein CueR(1). The promoter of cop A is more sensitive to copper than the other two copper-responsive promoter.RiboJ is an insulator commonly used in genetic circuits to prevent unexpected interactions between neighboring parts. Insulation with RiboJ may increase protein abundance. sfGFP) t increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding(2). sfGFP has been proven to be very useful for improved protein detection(3). Terminator L3SP22 is utilized for construction of a composite part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1121
Illegal SapI.rc site found at 364

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 <partinfo>BBa_K2555000 short</partinfo>
-BBa_K2555000 including the promoter of cop A, ribosome binding site, self-cleaving RNA ribozyme RiboJ, and protein coding region-sfGFP. In E.coli. as a  ATPase, cop A is  regulated by a copper-responsive protein CueR(1). The promoter of cop A is more sensitive to copper than the other two copper-responsive promoter.RiboJ is an insulator commonly used in genetic circuits to prevent unexpected interactions between neighboring parts. Insulation with RiboJ may  increase protein abundance. sfGFP) t increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding(2). sfGFP has been proven to be very useful  for improved protein detection(3). Terminator L3SP22 is utilized for construction of a composite part.
+BBa_K2555000 including the promoter of cop A, ribosome binding site, self-cleaving RNA ribozyme RiboJ, and protein coding region-sfGFP. In E.coli. As a  ATPase, cop A is  regulated by a copper-responsive protein CueR(1). The promoter of cop A is more sensitive to copper than the other two copper-responsive promoter.RiboJ is an insulator commonly used in genetic circuits to prevent unexpected interactions between neighboring parts. Insulation with RiboJ may  increase protein abundance. sfGFP) t increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding(2). sfGFP has been proven to be very useful  for improved protein detection(3). Terminator L3SP22 is utilized for construction of a composite part.
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