Difference between revisions of "Part:BBa K2586003:Design"
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===Design Notes=== | ===Design Notes=== | ||
In order to avoid the <i>PstI</i> restriction site, the Adenin at position 1135 was changed to a Thymin. | In order to avoid the <i>PstI</i> restriction site, the Adenin at position 1135 was changed to a Thymin. | ||
| + | |||
| + | ===Source=== | ||
| + | This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone. | ||
===References=== | ===References=== | ||
[https://www.ncbi.nlm.nih.gov/m/pubmed/25847720/ (Malone <i> et. al. </i> 2015)]: <i>EPSPS</i> gene amplification in glyphosate‐resistant <i>Bromus diandrus </i> | [https://www.ncbi.nlm.nih.gov/m/pubmed/25847720/ (Malone <i> et. al. </i> 2015)]: <i>EPSPS</i> gene amplification in glyphosate‐resistant <i>Bromus diandrus </i> | ||
Revision as of 11:09, 13 September 2018
Design Notes
In order to avoid the PstI restriction site, the Adenin at position 1135 was changed to a Thymin.
Source
This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.
References
(Malone et. al. 2015): EPSPS gene amplification in glyphosate‐resistant Bromus diandrus