Difference between revisions of "Part:BBa K2586006:Design"
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===References=== | ===References=== | ||
| − | ( | + | [https://www.ncbi.nlm.nih.gov/pubmed/17994626 (Herzberg <i> et. al.</i> 2007)] : SPINE: A method for the rapid detection and analysis of protein-protein interactions <i> in vivo</i>. <i>Proteomics</i>. 7:4032-4035 |
Latest revision as of 07:52, 13 September 2018
Strep-Tag-aroA: aroA linked to a Strep-Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1387
Illegal EcoRI site found at 74 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 74
Illegal NheI site found at 24
Illegal SpeI site found at 1388
Illegal PstI site found at 1402
Illegal NotI site found at 7
Illegal NotI site found at 1395 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 74
Illegal BamHI site found at 95 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1388
Illegal EcoRI site found at 74 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 74
Illegal XbaI site found at 16
Illegal SpeI site found at 1388
Illegal PstI site found at 1402
Illegal AgeI site found at 448
Illegal AgeI site found at 889 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 60
Illegal BsaI.rc site found at 1123
Design Notes
This part used (BBa_K2586007) as source.
Source
We added the Strep-Tag to (BBa_K2586007) to study protein-protein interactions of the EPSP-Synthase, which is encoded by the aroA gene.
References
(Herzberg et. al. 2007) : SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics. 7:4032-4035