Difference between revisions of "Part:BBa K2586004:Design"
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===References=== | ===References=== | ||
| − | ( | + | [https://www.ncbi.nlm.nih.gov/pubmed/17994626 (Herzberg <i> et. al.</i> 2007)] : SPINE: A method for the rapid detection and analysis of protein-protein interactions <i> in vivo</i>. <i>Proteomics</i>. 7:4032-4035 |
Latest revision as of 07:39, 13 September 2018
Strep-Tag-aroE: aroE linked to a Strep-Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1390
Illegal EcoRI site found at 74
Illegal PstI site found at 1232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 74
Illegal NheI site found at 24
Illegal NheI site found at 192
Illegal SpeI site found at 1391
Illegal PstI site found at 1232
Illegal PstI site found at 1405
Illegal NotI site found at 7
Illegal NotI site found at 1398 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 74
Illegal BamHI site found at 95 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1391
Illegal EcoRI site found at 74
Illegal PstI site found at 1232 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 74
Illegal XbaI site found at 16
Illegal SpeI site found at 1391
Illegal PstI site found at 1232
Illegal PstI site found at 1405
Illegal NgoMIV site found at 517 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 60
Design Notes
We added the Strep-Tag to (BBa_K2586003) to study protein-protein interactions of the EPSP-Synthase, which is encoded by the aroE gene.
Source
This part used (BBa_K2586003) as source.
References
(Herzberg et. al. 2007) : SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics. 7:4032-4035