Difference between revisions of "Part:BBa K2615007:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | + | We designed this part by point mutation. We changed the CAC(encoding His ) to GCG(encoding Ara) on the 29th site based on wild type Csy4. | |
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===Source=== | ===Source=== | ||
Revision as of 12:33, 11 September 2018
Csy4-H29A, the No.5 member of Csy4 family.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 377
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 93
Design Notes
We designed this part by point mutation. We changed the CAC(encoding His ) to GCG(encoding Ara) on the 29th site based on wild type Csy4.
Source
From