Difference between revisions of "Part:BBa K2586001:Design"
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===Source===
 
===Source===
  
−
This basic part consists of parts derived from PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).
+
This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain ''B. subtilis'' 168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).
  
 
===References===
 
===References===

Revision as of 06:47, 11 September 2018


GltT: glutamate and glyphosate transporter in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain B. subtilis 168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).

References