Difference between revisions of "Part:BBa K2586001"

Revision as of 06:42, 11 September 2018

GltT: glutamate and glyphosate transporter in B. subtilis

This part is coding for a glutamate and glyphosate transporter. The DNA sequence of the gltT gene is coding for the GltT transporter, which is responsible for glutamate uptake into the cell. We found that GltT is also involved in glyphosate uptake in the Gram-positive model bacterium Bacillus subtilis.

In the abscence of GltT causes the bacteria to develop a resistance against glyphosate, as less of this herbicide and antibiotic are transported into the cell. This means that this part has the potential to be involved in the development of glyphosate resistant detection and inactivation systems.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 1123
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2586001 short</partinfo>
-This part is coding for a glutamate and glyphosate uptake transporter.
+This part is coding for a glutamate and glyphosate transporter.
-The DNA sequence of the <i>gltT</i> gene is coding for the GltT transporter, who is responsible for glutamate uptake into the cell. However, gltT also has an affinity to glyphosate and is involved in its uptake as well.
+The DNA sequence of the <i>gltT</i> gene is coding for the GltT transporter, which is responsible for glutamate uptake into the cell. We found that GltT is also involved in glyphosate uptake in the Gram-positive model bacterium <i>Bacillus subtilis</i>.
 In the abscence of GltT causes the bacteria to develop a resistance against glyphosate, as less of this herbicide and antibiotic are transported into the cell. This means that this part has the potential to be involved in the development of glyphosate resistant detection and inactivation systems.