Difference between revisions of "Part:BBa K2573000"
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| + | ===Usage and Biology=== | ||
MetE codes for the methyl-transferase for methionine synthesis. Since methionine is required for E.coli growth, by introducing the MetE gene into plasmid desired for insert it can be used to differentiate between E.coli cells that have successfully taken up the insert and those that have not in a medium lacking methionine. | MetE codes for the methyl-transferase for methionine synthesis. Since methionine is required for E.coli growth, by introducing the MetE gene into plasmid desired for insert it can be used to differentiate between E.coli cells that have successfully taken up the insert and those that have not in a medium lacking methionine. | ||
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Revision as of 18:56, 10 September 2018
MetE Coding Device
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1864
- 1000COMPATIBLE WITH RFC[1000]