Difference between revisions of "Part:BBa I757011"
| Line 3: | Line 3: | ||
* first fragment of the split enzyme beta-lactamase | * first fragment of the split enzyme beta-lactamase | ||
| + | * purpose: split lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains | ||
| + | * works with second half of split lactamase (part BBa_I757012) | ||
| + | * this fragment contains stabilizing mutations | ||
* NgoMIV / AgeI protein fusion part | * NgoMIV / AgeI protein fusion part | ||
* iGEM Team Freiburg 2007 | * iGEM Team Freiburg 2007 | ||
Revision as of 16:15, 1 November 2007
bla_frag1(aa 26-199) Fusion Part
- first fragment of the split enzyme beta-lactamase
- purpose: split lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains
- works with second half of split lactamase (part BBa_I757012)
- this fragment contains stabilizing mutations
- NgoMIV / AgeI protein fusion part
- iGEM Team Freiburg 2007
- Synthetic DNA by GeneArt
- Part in pGA4 vector (AmpR, ColEI ori)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 535 - 1000COMPATIBLE WITH RFC[1000]