Difference between revisions of "Part:BBa K2629000:Experience"
| Line 10: | Line 10: | ||
<p>Experiments were done on a plasmid in which the probe has been inserted, thanks the Gibson technic, in psB1C3-BBa_J04450. </p> | <p>Experiments were done on a plasmid in which the probe has been inserted, thanks the Gibson technic, in psB1C3-BBa_J04450. </p> | ||
| − | <h1> Test A: <I> | + | <h1> Test A: <I> Is this part able to detect the target for which it has been designed ?</I> </h1><br> |
mettre schema | mettre schema | ||
| − | <h1> Test B: <I> | + | <h1> Test B: <I> Is this part able to detect specifically the target for which it has been designed ? </I> </h1> |
| + | |||
| + | <p> In order to evaluate the specificity of the detector part of BBa_K2629000, different “false target” sequences have been synthesized and tested with the detector. | ||
| + | An algorithm was made to give random sequences, more or less homologous to the original target with 5%, 15%, 25% and 50% randomly modified pairs of nucleotides (length is kept 36bp). <br> | ||
| + | The algorithm can be found here : </p><br> | ||
| + | Mettre schema | ||
Revision as of 09:18, 7 September 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2629000
Experiments were done on a plasmid in which the probe has been inserted, thanks the Gibson technic, in psB1C3-BBa_J04450.
Test A: Is this part able to detect the target for which it has been designed ?
mettre schema
Test B: Is this part able to detect specifically the target for which it has been designed ?
In order to evaluate the specificity of the detector part of BBa_K2629000, different “false target” sequences have been synthesized and tested with the detector.
An algorithm was made to give random sequences, more or less homologous to the original target with 5%, 15%, 25% and 50% randomly modified pairs of nucleotides (length is kept 36bp).
The algorithm can be found here :
Mettre schema
User Reviews
UNIQdc5e431c82186522-partinfo-00000000-QINU UNIQdc5e431c82186522-partinfo-00000001-QINU